TABLE 1.

VPg-interacting proteins identified using tandem affinity purification

VPg-containing complexes were purified from cells expressing TAP-MNV VPg expressed from either CdCl₂ or tetracycline-inducible promoters as described under Experimental Procedures. Complexes were separated by SDS-PAGE, and the identity of proteins was determined by mass spectrometry. Proteins present in complexes purified from cells expressing the TAP tag alone were considered non-specific interacting proteins and were removed from the analysis. The identified proteins are arranged based on their predicted molecular mass (Da) with the number of unique peptides and sequence coverage for each protein also listed. Where multiple accession numbers are available for any given protein, these are also listed.