TABLE 2.

Specific methane oxidation activities of apo M. capsulatus (Bath) pMMO when loaded with zinc first and then copper

Equivalent of zinc/copper added	Reductant	Activity	Percent of copper reconstituteda
		nmol methanol/min·mg
10 zinc/3 copper	Duroquinol	5.1 ± 0.9	17 ± 3
	NADH	7.7 ± 0.9	18 ± 2
10 zinc/5 copper	duroquinol	4.1 ± 0.2	14 ± 1
	NADH	4.8 ± 0.6	11 ± 2

^a The activities of the copper-reconstituted membrane-bound pMMO were 30 ± 2 (duroquinol) and 43 ± 3 (NADH).