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. 2013 Jul 11;17(9):1146–1159. doi: 10.1111/jcmm.12094

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© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine

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Fig. 4 — Elevated expression of Tm1α/2β proteins in lens epithelial cells (LECs) transfected with miR29a and 29c inhibitors. Lens epithelial cells isolated from Shumiya Cataract Rats (SCR) with cataract (+) or SCR without cataract (−) were cultured and processed for transfection with miRNA inhibitors as described in Materials and methods. At a pre-defined time, cellular extracts were prepared and subjected to protein expression analysis using antibody specific to Tm1α/2β. Immunoblotting experiments revealed greater abundance of Tm1α/2β protein in LECs transfected with miR29a and 29c inhibitor. Representative immunoblots show modulated expression of specific miRNA targeted gene Tm1α/2β. Blotted membranes were probed with antibody specific to Tm1α/2β and reprobed with β-actin antibody as internal and protein loading control. Histograms represent normalized mean results ± SD. *P < 0.05 is considered to be statistically significant. Non: No transfection; NC: Negative control; PC: Positive control.