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. 2013 Aug 13;17(9):1188–1193. doi: 10.1111/jcmm.12098

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© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine

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Fig. 3 — Effect of PPARγ suppression on osteogenic differentiation of hADSCs. Human ADSCs were transfected with 50 nmol/l PPARγ2 siRNA for 48 hrs, and cultured in osteogenic induction medium for another 0, 2, 6 or 12 days (corresponding to 2, 4, 8 or 14 days after siRNA transfection) to determine the mRNA level of (A) BMP2, (B) Runx2, (C) ALP and (D) OC. (E) Cells were induced to differentiate osteogenically as described in Materials and methods. The level of matrix mineralization was determined by alizarin red S staining 14 days after siRNA transfection (n = 3, **P < 0.01 compared with control and mock). (F) The role of PPARγ in lineage differentiation of hADSCs and the effect of PPARγ suppression as concluded from this study.