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. 2014 Jul 22;11:33. doi: 10.1186/1742-4682-11-33

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Copyright © 2014 Polidori and Rowley; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Illustration of the back-extrapolation method for 2 different clearance rates. Note: The back-extrapolation for each case was performed by fitting the simulated data from t = 2 to 5 minutes to a mono-exponential decay and then back-extrapolating to t = 0 [3].