Fig. 4.

Efficiency of transfection and effects of anti- and pre-miR oligonucleotides on apoptosis and cell cycle. (A) Suppression of miR-135b expression by anti-miR-135b as detected by TaqMan qRT-PCR. Compared with negative control, miR-135b was reduced by 70.5 ± 16.1% in HCT-116 cells and by 60.0 ± 17.3% in DLD-1 cells. (B) Effect of miR-215 on apoptosis of HCT-116 cells. Transfection of 10 nM pre-miR-215 significantly increased the number of apoptotic cells from 14.6 ± 2.1% to 36.2 ± 5.1%. C. Staining with propidium iodide for cell cycle analysis. miR-215, miR-375, miR-378 and miR-422a significantly increased the number of cells in G0–G1 phase and reduced S-phase cells in HCT-116 (wt-p53) cell line. Results from three independent experiments (*t-test significant at P < 0.05, **P < 0.01).