Fig. 5.

Viability of DLD-1 and HCT-116 cells determined with MTT assay. Cells were transfected with 10 nM precursors or 30 nM inhibitor of particular miRNAs, resp., and cell viability was measured by MTT test 48 hrs after transfection. (A) miR-375 decreased viability of DLD-1 cells by 10.0 ± 9.7% and HCT-116 cells by 32.9 ± 6.9%. (B) miR-215 decreased viability of DLD-1 cells by 19.7 ± 7.1% and HCT-116 cells by 21.7 ± 0.3%. (C) Inhibition of miR-135b expression led to decrease in viability of DLD-1 cells by 27.4 ± 19.2 % and HCT-116 by 16.0 ± 11.7%. Results from three independent experiments (*t-test significant at P < 0.05, **P < 0.01).