Fig. 3.

DC adherence to plastic tested in a ‘scratch assay’. DC were matured with TNF-α (DC1); TNF-α, IL-1α, IL-6, PGE2 (DC2); TNF-α, IL-1β, IFN-γ, PGE2, R848 (DC3); IFN-γ, LPS (DC4); IFN-γ, R848 (DC5) or were cultured without maturation (DC6). Scratch assay was performed after 24 hrs of maturation. The contrast of all pictures has been increased artificially and equally to facilitate the observation of the differences among DC types. (A) Scratch assay, 0 hr: a scratch without cells created in a DC monolayer by a pipette tip. The scratch is marked with an arrow on its both ends. (B) Scratch assay, after 6 hrs: adherent cells do not fill in the scratch, whereas migrating cells do. Each scratch is marked with an arrow. Pictures represent results obtained from four different donors, magnification of 400.