Fig. 4.

DC in vitro migratory capacity. DC were matured with TNF-α (DC1); TNF-α, IL-1α, IL-6, PGE2 (DC2); TNF-α, IL-1β, IFN-γ, PGE2, R848 (DC3); IFN-γ, LPS (DC4); IFN-γ, R848 (DC5) or were cultured without maturation (DC6). Box plots represent (A) spontaneous DC migration (no CCL21 added), (B) migration towards 100 ng/ml CCL21, (C) the ratio of CCL21-induced migration/spontaneous migration and (D) CCR7 expression by DC. Data are presented as the median (▪), 25–75% quantiles (box), and non-outlier range (whiskers) of three (A, B, C) or five (D) independent experiments, two donors per group. Marker ▲ indicates significant difference from all groups not indicated by this marker, P < 0.05, Wilcoxon matched pair test.