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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Biochem J. 2012 Aug 15;446(1):113–123. doi: 10.1042/BJ20120112

Figure 7.

Figure 7

Overexpression of CUG-BP1 in esophageal cancer cells abrogates increased sensitivity to apoptosis following down-regulation of survivin. A: Representative immunoblots and densitometry analyses of survivin and CUG-BP1 after the co-transfection of siSurvivin plus CUG-BP1 cDNA in TE7 cells. B: Representative morphologic analyses of un-induced (top panels) and camptothecin-induced (bottom panels) TE7 cells after a. C-siRNA + vector, b. C-siRNA + CUG-BP1 O/E, c. siSurvivin + vector, and d. siSurvivin + CUG-BP1 O/E. Cells were transfected with appropriate siRNA and cDNA for 48 hours and then exposed to camptothecin for 6 hours. C: Percentage of apoptotic cells after co-transfection. Cells were prepared as described in (B) and apoptotic cells were counted and graphed as a percentage of total cells. *p < 0.05 compared to C-siRNA + CUG-BP1 O/E and siSurvivin + CUG-BP1 O/E; +p < 0.001 compared to C-siRNA + CUG-BP1 O/E and siSurvivin + CUG-BP1 O/E.