Skip to main content
. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Biochem J. 2012 Aug 15;446(1):113–123. doi: 10.1042/BJ20120112

Table 1.

Primers used for preparation of biotinylated transcripts

Sequence Region
CR (T7) 5′-CCATTAACCGCCAGATTTGA-3′
  5′-TCAATCCATGGCAGCCAGCT-3′
66–619
FL (T7) 5′-TGTCTTGAAAGTGGCACCAG-3′
  5′-ACTTTCCAGGATGGCAGTTG-3′
773–2576
F1 (T7) 5′-CATGGATTGAGGCCTCTGG-3′
  5′-CCTCCCTCACTTCTCACCTG-3′
610–918
F2 (T7) 5′-AGGGAGGAAGAAGGCAGTGT-3′
  5′-AGAGGAGCCAGGGACTCTGT-3′
912–1199
F3 (T7) 5′-CAGAGTCCCTGGCTCCTCTA-3′
  5′-TAGGCCACTGCCTTTTTCTG-3′
1181–1458
F4 (T7) 5′-AGGCAGTGGCCTAAATCCTT-3′
  5′-TGACCTCCAGAGGTTTCCAG-3′
1446–1723
F5 (T7) 5′-ATCTCGGCTGTTCCTGAGAA-3′
  5′-CAAGCGCCCAGAGACAACT-3′
1723–1903
F6 (T7) 5′-CTTGCCAGAGCCACGAAC-3′
  5′-CCATTTACAGACTGACACAAATATCA-3′
1900–2136
F7 (T7) 5′-GTCAGTCTGTAAATGGATACTTCACT-3′
  5′-ATGTCGAGGAAGCTTTCAGGT-3′
2121–2380
F8 (T7) 5′-CCTGAAAGCTTCCTCGACAT-3′
  5′-AAAACCACATGAGACTTTATTGG-3′
2361–2695