Amplified fragments using the An. funestus group species-specific PCR of Koekemoer et al. (2002). Lanes 1–10, An. longipalpis Type C; lanes 4 and 9, no amplification; lane 2, shows a faint large fragment; however, when the sample was repeated, the large fragment was clearly visible; lanes 11–16, Type A; lane 17, An. vaneedeni; lane 18, An. funestus (no amplification on this gel due to human error, expected size: 500 bp); lane 19, An. rivulorum; lane 20, An. parensis; lane 21, An. leesoni; lane 22, DNA extraction negative control; lane 23, PCR mastermix negative control with no template; and lane 24, Hyperladder IV (100 bp) molecular marker.