Table 3.

Variation of ELISA conditions for further optimization of assay sensitivity (n = 8, m = 3).

Antibody Dilution	Coating Antigen Dilution	IC50 [μg/kg]	Working Range [μg/kg] (20%–80% of Max. Signal)	LOD [μg/kg]
1:20,000	1:5,000	8.10	2.56–25.66	1.27
1:20,000	1:10,000	8.25	2.35–29.15	2.34
1:20,000	1:15,000	5.45	2.06–14.45	1.79
1:40,000	1:5,000	52.05	4.78–567.38	4.24