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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Nature. 2014 Apr 28;510(7506):556–559. doi: 10.1038/nature13295

Figure 4. Brca1 Exon11 regulates Tus/Ter-induced HR.

Figure 4

(a) Brca1 gene in Brca1fl/Exon11 ES cells. Brca1Exon11 encodes Δexon11 product. Cre converts Brca1fl to exon11-deleted Brca1Δ allele. Symbols as in Fig. 3. PCR primers a, b, and d shown. (b) Tus- and I-SceI-induced HR in Brca1fl/Exon11 and Brca1Δ/Exon11 6xTer-HR cells (three independent clones each). Mean of triplicate samples, n=4. Error bars: s.e.m. t-test Brca1fl/Exon11 vs. Brca1Δ/Exon11, in all 6 panels P<0.005. (c) Upper panel: endogenous Brca1 immunoblot in Brca1fl/Exon11 and Brca1Δ/Exon11 ES cells. *: background band. Lower panel: ß-actin loading control. (Source Data 7.) (d) PCR genotyping of Brca1fl/Exon11 and Brca1Δ/Exon11 clones from panel b. P: untargeted Brca1fl/Exon11. E: “empty” (no DNA) control. Brca1fl product: 531 bp. Brca1Δ product: 621 bp.