Extended Data Figure 6. Southern blot analysis of Tus/Ter- and I-SceI-induced HR products in Brca1Δ/BRCT 6xTer/HR cells.
a, Structure of the 6xTer/HR parental reporter, and major STGC or LTGC HR products (assuming two-ended breaks). Elements as shown in Fig. 2a. b, Southern blot analysis of Tus-induced and I-SceI induced HR products in Brca1Δ/BRCT 6xTer-HR cells. P: un-rearranged reporter; STGC and LTGC as shown. SN: STGC accompanied nondisjunction with retention of parental donor reporter; LN: LTGC accompanied nondisjunction with retention of parental donor reporter. B: BglII digest. BI: BglII + I-SceI digest. Membranes probed with full length GFP cDNA. Panels underneath two SN events and one LN event show that re-cloning does not separate the two reporters, confirming that the cell contains two copies of the reporter (consistent with nondisjunction).