(A). Effect of ASHMI™ and three individual herb extracts (in concentrations equivalent to that in ASHMI™ ) on acetylcholine (ACh)-induced contraction of ASM in tracheal rings from sensitized/challenged BALB/c mice. Acetylcholine contractions are expressed as a percent of 60 mM KCl contractions and the graph shows mean ± SEM. (n=3 tracheal ring per treatment) (***, p<0.001 significantly different from the control group ) The concentrations of ASHMI, S. flavescens, G. lucidum, and G. uralensis were 100, 45, 35, and 20 μg/ml respectively. (B). Representative myography tracings of control (PBS) (upper tracing) and S. flavescens-treated (lower tracing) tracheal rings from sensitized/challenged BALB/c mice.