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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Stem Cell Res. 2014 Apr 22;13(1):154–163. doi: 10.1016/j.scr.2014.04.005

Figure 5. Castration induces proliferation of BrdU labeled cells.

Figure 5

(A) Clusters of BrdU labeled cells (arrows) were observed in mice labeled and castrated as described in Figure 4. Similar clusters were never observed in the intact controls. Co-staining for BrdU and CK5 identified the cluster of labeled cells as basal cells. (B and C) Staining for Ki67 in mice labeled and castrated as described in Figure 4 revealed increased proliferation of BrdU labeled cells. (B) Immunofluorescence staining for BrdU (red), Ki67 (green) and DAPI (blue) shows co-localization of BrdU with Ki67. This is quite uncommon in intact controls. Quantitative analysis of co-staining for Ki67 and BrdU confirms co-staining is significantly increased castrated animals (C) (n=5).