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qPCR validation of gene expression in TCDD-CD4+ cells. Up-regulated genes of interest identified on a DNA microarray chip were validated by qPCR using gene-specific primers and normalized for β-actin expression. Results are expressed as fold increase in gene expression in TCDD-CD4+ cells relative to VEH-CD4+ cells; n= 3 per treatment (n = 2 pooled mouse spleens).
