Figure 3.

TLR2-dependentPSA expansion of CD39⁺ CD4 T cells is tissue site-specific. Wild-type and TLR2^KO mice were treated with the same regimen as described in Fig 1A setting. At the peak of disease, total leukocytes were isolated from cervical lymph nodes (CLNs), mesenteric lymph nodes (MLNs), spleen and whole blood and flow cytometry analysis was performed. Oral PSA administration stimulates CD39 expression on CD4 T cells via TLR2 at both (A) CLNs and (B) MLNs. CD4 T cells were pre-gated and CD39 expression level was measured in (A) and (B). Depicted are the typical histograms for each indicated treatment group from two independent experiments (n = 3–10, per group). (C) TLR2-dependent PSA induction of CD39 signal on CD4 T cells is restricted to CLNs and MLNs. Frequencies of CD39⁺ subsets among CD4 T cells were quantified for the indicated tissue sites. Depicted are combined representative results of two independent experiments (n = 6–10, per group). P value was calculated by two-tailed Student’s t test. (D) PSA does not influence CD39 signal on CD19 B cells. Frequencies of CD39⁺ subsets among CD19 B cells were quantified for both CLNs and MLNs. Combined representative results of two experiments are shown (n = 6–10, per group).