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. 1979 Dec;76(12):6331–6335. doi: 10.1073/pnas.76.12.6331

Single-strand binding protein enhances fidelity of DNA synthesis in vitro.

T A Kunkel, R R Meyer, L A Loeb
PMCID: PMC411858  PMID: 230506

Abstract

The effect of Escherichia coli single-strand binding protein on the accuracy of in vitro DNA synthesis has been determined by using two independent methods. By using the synthetic polynucleotide poly[d(A-T)] and measuring dGTP misincorporation or by using phi X174 DNA and measuring nucleotide substitutions, we found that binding protein increases the fidelity of DNA synthesis by as much as 10-fold. This increase is observed with DNA polymerases of divergent sources and is progressive with increasing concentration of binding protein. The increased accuracy observed with DNA polymerases lacking a 3' leads to 5' exonuclease points to a mechanism other than augmented proofreading. In accord with the properties of single-strand binding proteins, it is suggested that increased fidelity is a result of enhanced base selection by the DNA polymerase, resulting from increased rigidity of the template due to its interaction with binding protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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