Figure 3. Enrichment analysis of the consensus cytokine signature emphasizes a macrophage-directed inflammatory phenotype.
(A) Significance of lineage-specific enrichment scores for consensus cytokine expression obtained by GSEA among Immune Response in silico (IRIS) transcriptional profiles. (B) Hierarchy of enriched intercellular cytokine-receptor interactions. Flow cytometric confirmation of increased (C) absolute and (D) relative CD68+ macrophage abundance among endometriosis patient populations. Increasing CD68+ counts among patient subpopulations C1 and C2 are shown for comparison. **P<0.01 Wilcoxon rank-sum test; †P<0.05, †††P<0.001, linear trend test. (E) Normalized concentration of secreted cytokine in media conditioned from isolated peritoneal macrophages. Shown are 13 differentially secreted cytokines (FDR<0.05 Wilcoxon rank-sum test, two-fold increase) from eight control and eight untreated donors.