Figure 4.

Yeast prefoldin stimulates transcription elongation by favouring chromatin dynamics. A subset of canonical prefoldin subunits, including PFDN1, 4, 5 and 6, binds transcribed genes in a transcription-dependent manner and contributes to transcription elongation. This effect is maximal in long genes, where prefoldin stimulates cotranscriptional histone eviction. Prefoldin binding is absent in the promoter region and is maximal in the 3′ end of the gene body to correlate with the presence of Ser2-hyperphosphorylated RNA polymerase II.