Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Nutr Biochem. 2014 Apr 18;25(8):868–874. doi: 10.1016/j.jnutbio.2014.03.017

Figure 3.

Figure 3

Co-treatment with DHA prevents palmitate-induced alterations in FoxO3 localization. A) Treatment (4 h) with 500 µmol/L PA (P) and/or 100 µmol/L DHA (D) does not alter FoxO3 protein in total cell lysates (n=3/group). PA decreases cytosolic (B) and increases nuclear FoxO3 protein (C), while co-treatment with DHA (PD) prevents the responses (*p<0.01 versus all other groups, n=4/group). D) Representative western blots show markers of cytosolic (GAPDH), nuclear (histone H1), and cell membrane (Na+-K+ ATPase) proteins to confirm the purity of cell fractions.