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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2012 Sep 20;53(11):2081–2091. doi: 10.1016/j.freeradbiomed.2012.09.011

Fig. 10.

Fig. 10

Cellular generation of O2•− detected by CT02-H. The O2•− generation was stimulated by adding menadione (100 μM) to the cell suspension (3.8 × 105 (■) or 1.2 × 105 (●) cells/ml in a 40-μl EPR capillary tube) containing CT02-H (0.2 μM) and DTPA (100 μM) in DMEM. EPR spectra were recorded 5 min after the addition of menadione. SOD (200 U/ml) (△) was used to verify the O2•− generation. The concentration of CT02-H was quantitated by comparing the double integral of the EPR signal of each radical with its predetermined concentration (i.e., 0.2 μM).