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Combined EF loop clustering of corresponding systems. Stacked bars represent combined clusters from two separate simulations of the wild-type CH3 domain (blue), the double mutant Q418L/S424T (red), the stabilized, ‘randomized’ variant stem(0) (green) and the non-stabilized, randomized variant stem-(0) (purple). RMSD (in nm) of cluster central member structures with respect to the central member structure of cluster 1 are shown on top of bars.
