Table 2.

Reference and target gene efficiency determination and amplicon melting points, r ² values are regression coefficients for the curves

Candidate reference genes	Slope of the curve	r 2 on the slope	PCR efficiency	Melting temperature (°C)
proC	−3.78	0.997	1.84	80.0
gyrB	−3.85	0.998	1.82	79.5
rplD	−3.86	0.998	1.82	75.5
rho	−3.68	0.997	1.87	81.0
rpoA	−3.89	0.996	1.81	76.5
ftsZ	−3.85	0.998	1.82	79.0
recA	−3.83	0.998	1.82	81.0
sodA	−3.97	0.996	1.79	79.5
Target gene
icaA	−3.92	0.995	1.80	80.5