Table 5.

Candidate reference and target genes, primers and amplicon sizes

Candidate reference genes	Function	Primer sequence (5’-3’)	Expected size (bp)
proC	Pyrrolidine-5-carboxylate reductase	proC-F gccgaatacaaatgcgcacg	180
		proC-R aaaaatgcagggccacttcc
gyrB	DNA gyrase B subunit	gyrB-F gcgtccgttgattgaagcg	240
		gyrB-R aacgtcacttgcaacatcgc
rplD	50S ribosomal protein L4	rplD-F gcctaagaaaatgcgtcg	237
		rplD-R ccttctggtgttgtgattg
rho	Transcription termination factor Rho	rho-F cacgtaaaagttgctgaattg	215
		rho-R cctgcttcgatatttctgg
rpoA	DNA-directed RNA polymerase sigma factor	rpoA-F ctatcatcattaccaggtgc	231
		rpoA-R caaaatttcaacatcactgtc
ftsZ	Cell division protein ftsZ	ftsZ-F gtccattcagtttcgaagg	254
		ftsZ-R catgattgttttaacgtcagc
recA	Recombinase A	recA-F gcattaggtgtagatattgataac	228
		recA-R ggctgcagaaagtttacgc
sodA	Superoxide dismutase	sodA-F cgcaaacttagacagcgtacc	227
		sodA-R caacaagccaagcccaacc
Target Gene
icaA	N-acetylglucosaminyl-transferase	icaA-F ttgcccaccttgtgcccacc	178
		icaA-R tgaggctgtagggcgttggga