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Center, negative-stain TEM image of a complex between RNA polymerase II and green fluorescent protein (GFP). Upper left, crystal lattice; lower left, Bragg spots calculated after applying a Fourier transform showing at least third-order spots. The high lattice quality correlated with diffraction to 4.0 Å resolution at LCLS (right panel). Modified with permission from Stevenson et al. (2014 ▶).
