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Crystallization of Fab 1-69/A27. (a) Hits from initial screening at 30 mg ml−1 in 2.0 M AmSO4, 5% MPD, 0.1 M MES pH 6.5. (b) Crystals in 2.0 M AmSO4, 5% MPD, 0.1 M Tris pH 8.5 obtained by MMS at 16 mg ml−1. (c, d) Results from refinement screening at 16 mg ml−1 with seeds from (a) and AmSO4 concentrations of 2.07 M (c) and 2.19 M (d).
