Table 1. Primers used in gene-cloning experiments.

Protein construct/vector	Primers
VapA-full/pET-30a(+)	FWD 5-GGAATTCCATATGCCGTTCTTGATTCCCGG-3
	REV 5-CCGCTCGAGGGCGTTGTGCCAGCTACCAGA-3
VapD-full/pET-30a(+)	FWD 5-GGAATTCCATATGCAGGAGCTAGCTGGCACC-3
	REV 5-CCGCTCGAGCTCCCACCCGCCAGTGCC-3
VapD-core/pET-22b	FWD 5AAGGAGATATACATATGGCCATTCCTGAAGATAAAGAG-3
	REV 5-GGTGGTGGTGCTCGAGCTCCCACCCGCCAGTG-3
VapD-core(L100M/V123M)-SeMet/pET-22b	FWD_L100M 5-GCGGCCGCAACATTCATGAATATCAACTTTTTC-3
	REV_L100M 5-GAAAAAGTTGATATTCATGAATGTTGCGGCCGC-3
	FWD_V123M 5-GGTGCTGCTGGGACAATGTCGGGGATCGGTGGT-3
	REV_V123M 5-ACCACCGATCCCCGACATTGTCCCAGCAGCACC-3