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. 2014 Jul 25;70(Pt 8):2186–2196. doi: 10.1107/S1399004714013315

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© Kelleher et al. 2014

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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The SmVAL4 monomer. (a) A Coomassie-stained SDS gel reveals the purity of the SmVAL4 sample and its monomeric mass of ∼20 kDa. Lane M contains molecular-weight marker (labeled in kDa). (b) SEC-MALS analysis reveals that SmVAL4 is an ∼20 kDa monomer in solution. (c) Fit of N-glycosylated Asn118 and proximal residues into a 2F _o − F _c electron-density map (gray) calculated from the refined model of SmVAL4 and contoured at 1.2σ. NAG, N-acetyl-d-glucosamine. (d) Topology of the SmVAL4 structure.