Figure 2. Validating the specificity of the UBR4 antibody employed in the current study.

(A) The human UBR4 immunogen sequence that was used to generate the rabbit polyclonal UBR4 antibody and its alignment with murine UBR4. Nucleotides that are identical between the two sequences are highlighted in yellow. (B) Western blot analysis of UBR4 protein levels in mock-transfected N2A control, N2A cells with siRNA knockdown of UBR4, and C57BL/6J mouse SCN. All lanes expressed a band that was greater than 300 kDa (corresponding to UBR4, predicted size ∼570 kDa), the intensity of which was greatly diminished upon UBR4 siRNA knockdown. Actin was used as the loading control.