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. 2014 Aug 1;9(8):e103103. doi: 10.1371/journal.pone.0103103

Figure 5. Light inducibility of UBR4 in the SCN depends on time-of-day.

Figure 5

(A) Representative micrographs illustrating the expression of UBR4 in the rostral-middle section of the SCN following a light pulse. Light pulses (LP) (15 min, 100 lux) were administered at CT 6, 15 and 22 and tissues were harvested 4 h later. Dark (DD) controls were killed at the same time without prior light treatment. (B) Quantification of mean UBR4 intensity in individual cells in the rostral-middle section of the SCN after a light pulse given at the indicated time. Values were normalized to background staining. y-axis represents mean intensity of UBR4 staining in individual cells in grayscale intensity units. x-axis represents the CT when the light pulse was administered. (C) Quantification of the number of UBR4-expressing cells in the rostral-middle section of the SCN after a light pulse given at the indicated time. y-axis represents number of counted UBR4-positive cells. n = 4 animals per time point. *p<0.05 LP vs. DD control. (Scale bar  = 50 µm).