Table 1. Primers for MSP of the ABCG1, GALNT2 and HMGCR genes.

Group	Forward primer(5′to3′)	Reversr primer(5′to3′)	Product size (bp)	Tm
ABCG1 M	5′ ATTTGTATTGTGATATCGACGAGAC 3′	5′ CTTACCTCCTCGATTCTAAACGTAC 3′	251	54
ABCG1 U	5′ AGATTTGTATTGTGATATTGATGAGAT 3′	5′ AACTTACCTCCTCAATTCTAAACATAC 3′	251	48
GALNT2 M	5′ TTATAAGATAGATCGTTTTTTTGTATC 3′	5′ CCGCTAATATCGATTTTATTTAT 3′	263	48
GALNT2 U	5′ ATGTTATAAGATAGATTGTTTTTTTGTATT 3′	5′ AACCCACTAATATCAATTTTATTTAT 3′	263	46
HMGCR M	5′ TATAAGAGAGAGAGACGTAGGTGATC 3′	5′ CCCGTACTCGTCCTAACTATAATAA 3′	290	55
HMGCR U	5′ GTATATAAGAGAGAGAGATGTAGGTGATT 3′	5′ TAACCCATACTCATCCTAACTATAATAA 3′	290	54

MSP: methylation-specific polymerase chain reaction; M: methylation-specific primers; U: unmethylation-specific primers.