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. 2014 Aug 1;9(8):e103294. doi: 10.1371/journal.pone.0103294

Figure 2. Specificity of single domain antibodies to different influenza antigen reference reagents.

Figure 2

ELISA showing binding of 18 purified single domain VHH antibodies to influenza reference reagents prepared from different viral subtypes (A) ELISA comparing binding of purified VHH antibodies at 30 µg/ml against A/California/07/2009 (H1N1pdm09), A/Brisbane/59/2007 (seasonal H1N1),A/Anhui/01/2005 (H5N1) (B) A/Vietnam/1194/2004 (H5N1) (C) A/Indonesia/05/2005 (H5N1) (D) A/HongKong/05/2005 (H5N1) (E) A/Singapore/1/57 (H2N2) (F) A/Hong Kong/1073/99 (H9N2). Antibodies R2b-E8, R2b-D9, R1a-A5, R1a-B6 and R2a-G8 were also positive on A/turkey/Turkey/01/2005 (H5N1), A/Duck/Sing-Q/119-3/97 (H5N3), A/mallard/Eng/727/2006 (H2N3) (data not shown). In addition all antibodies were negative on B virus control (B/Brisbane/60/2008) and on the group 2 strain A/Brisbane/10/07 (H3N2) (data not shown). Control represents a non-binding purified VHH antibody. Binding was measured in duplicate and the average OD 450 nM was plotted against a serial dilution of antibody from 30 µg/ml to 0.001 µg/ml (B-F).