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. 2014 Aug 1;9(8):e103294. doi: 10.1371/journal.pone.0103294

Table 3. Antibody classification.

Clone Antibody neutralisation category1 HI titre (µg/ml) H1N12 H1 (D18-I530)3 KD (nM) HA1 (H1N1) (D18-R344)3 KD (nM) HA4 P66-I282 HA4 L193-K225 HA4 D363-G478 Low pH treated HA 5 Neutral pH treated HA 5
R1a-G5 R 1.56 5.85 15.6 + - - + +
R2b-D8 N - - - - - + ++ +
R2b-E8 C - 1.74 - - - - - +
R2b-D9 C - 0.18 - - - - - +
R1a-F4 R 2.08 6.46 4.74 + - - + +
R1a-A5 C - 1.87 - - - - - +
R1a-C5 R 10.40 37.57 38.40 + + - + +
R1a-E5 R - 0.79 - - - - - +
R1a-F5 N - 10.00 6.06 + - - + +
R1a-B6 C - 0.86 - - - - - +
R1a-E6 N - 9.97 36.00 + - - + +
R1a-G6 R 1.04 4.83 4.62 + - - + +
R2a-E8 R - 0.79 - - - - - +
R2a-G8 C - 3.55 7.66 - - - - +
R2a-B9 R - 0.16 - - - - - +
R2a-F9 R 1.04 78.80 78.80 + - - + +
R2a-G9 R 1.30 22.14 68.70 + - - + +
R2a-H9 R 1.30 2.77 468.00 + - - + +
control - - - - - - - - -
1

Antibody neutralisation category, R is H1N1 restricted neutralisation activity, C refers to a cross-neutralising antibody, N is non-neutralising antibody

2

Hemagglutination inhibition (HI) titre was given as the minimum dilution of antibody at which inhibition of agglutination of turkey erythrocytes was observed (in µg/ml of purified antibody). Values are given as an average of three independent assays. Laboratory adapted A(H1N1) strain (X-181) was used.

3

Single cycle affinity determination on full length HA (D18-I530) and globular head HA1 domain (residues D18-R344) derived from A/California/06/2009 (H1N1pdm09). Affinity is given in nM.

4

Binding to phage displayed fragments of HA as indicated in ELISA (see Figure 5B).

5

ELISA on HA of A/California/07/2009 (H1N1pdm09) treated with low pH and neutral pH (see Figure 5A).