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. 2014 Aug 1;9(8):e103566. doi: 10.1371/journal.pone.0103566

Figure 3. SDS-PAGE and immunoblot of the purified mouse epididymal protein.

Figure 3

Aliquots (100 µg) of immunoreactive pools A, B and C obtained by ion exchange chromatography and epididymal extracts were electrophoresed in a 7.5% polyacrylamide gel (A) and transferred to a PVDF membrane for immunoblotting with mAb TRA 54 (B). A strongly immunoreactive band of ∼65 kDa was detected in pool A (arrowhead in panel B), but not in pools B and C. The corresponding protein band in the polyacrylamide gel stained with Commassie blue (arrowhead in panel A) was excised and the protein digested with trypsin. Control immunoblots without mAb TRA 54 showed no bands (not shown). MM – molecular mass markers.