Skip to main content
. 2014 Aug 1;9(8):e103566. doi: 10.1371/journal.pone.0103566

Figure 5. Albumin mRNA transcripts detected by RT-PCR.

Figure 5

(A) RT-PCR detected albumin mRNA in mouse testis and epididymis (and all regions of the latter). In these assays, mouse liver was used as a positive control and fibrous cartilage from mouse tail was used as a negative control. The PCR products were analyzed by electrophoresis in 3% agarose gels and included a 100 bp DNA ladder. (B) The housekeeping gene was used to check the intactness and quality of total cDNA. The forward and reverse primers for PCR were: 5′-CTTGCTGCAGACATGGTC-3′ and 5′-GCAATCCTGCTAGACTTG-3′, respectively. The blank was used as a control RT-PCR reaction. The PCR products were analyzed by electrophoresis in 3% agarose gels and included a 100 bp DNA ladder. (C) The size of the albumin PCR fragment was confirmed by digestion using the restriction enzyme HinfI. The digestion fragments were analyzed by electrophoresis in 3% agarose gels and included a 100 bp DNA ladder.