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. 2014 Aug 1;9(8):e103624. doi: 10.1371/journal.pone.0103624

Figure 1. CHIKV infection increases miR-146a expression and decreases TRAF6, IRAK1, and IRAK2 in primary human synovial fibroblasts.

Figure 1

Expression of miR-146a was increased upon CHIKV infection. (A). Human primary synovial fibroblasts were infected with CHIKV at MOI of 2 and cells were harvested 32 hours post infection for RNA isolation and protein lysate preparation. Expression levels of miR-146a were determined by qRT-PCR with TaqMan primers and probes specific for miR-146a. Expression level of RNU 24, an endogenous control, has been used as normalizer and the results are shown as fold changes compared to controls. (B) Western blot analysis showing decrease in the protein expression levels of TRAF6 in CHIKV infected primary human synovial fibroblasts. (C) Graph bars are showing densitometry analysis of TRAF6 normalized with housekeeping gene β-tubulin by ImageJ software. (D). Western blot analysis showing decrease in protein expression levels of IRAK 1 and IRAK 2 in CHIKV infected primary human synovial fibroblasts compared to uninfected controls. (E) Graph bars representing densitometry analysis for expression levels of IRAK1 and IRAK2 normalized with β-tubulin. All the experiments were independently repeated three times and shown as mean ± SEM. * above bars are representing the p value≤0.05 as level of significance, n = 3 (*for p value≤0.05).