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. 2014 Aug 1;9(8):e103868. doi: 10.1371/journal.pone.0103868

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© 2014 Rizwani et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot showing overexpressed profilin 1 and its mutants (41 KDa), endogenous profilin 1 (14 kDa) and actin (42 KDa). (B) Phase contrast image showing well spread out monolayer of transfected MCF7 cells (upper panel) and rounded nuclei alone at 2 min and 10 min (middle and bottom panels). Magnification-20×. Flow cytometric analysis showing green fluorescent positive nuclei from (C) EV, (D) PFN-WT, (E) PFN-S137A, (F) PFN-R74E and (G) PFN-H133S clonal populations.