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. 2014 Aug 1;9(8):e103868. doi: 10.1371/journal.pone.0103868

Figure 7. MMP2/MMP9 activity increases with phosphorylation of profilin 1 in MCF7 cells.

Figure 7

(A) Gelatin zymography showing minimal MMP2 activity in serum-free media at 24 hr in all the clones. 200 nM PDBu treatment induced MMP2 and MMP9 activity significantly. PFN-WT expressed highest MMP2 activity whereas PFN-S137A the least. MMP9 activity was most expressed in PFN-R74E and PFN-WT followed by PFN-H133S. PFN-S137A showed the least MMP9 activity comparable to EV. (B) Nuclear translocation of profilin 1 and its mutants increases with time upon exposure to 200 nM PDBu when compared to serum starved cells except for PFN-S137A that remains constant throughout. PFN-S137A was comparable to EV.