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. 2014 Aug 1;9(8):e104080. doi: 10.1371/journal.pone.0104080

Figure 4. Real-time polymerase chain reaction (RT-PCR) on HVCN1, PMAIP1, ARRB1 and DOM3Z in blood neutrophils obtained from CF patients pre- and post-therapy confirming microarray expression data.

Figure 4

A) Data were generated by comparing 5 healthy controls and 5 CF patients before and after therapy setting gene expression in healthy controls at 1. HVCN1 was less expressed in CF patients as compared to controls and its expression increased after therapy to values above those of controls. ARRB1 and PMAIP1 in CF patients before therapy showed similar levels of the control subjects but nevertheless increased after therapy above values of controls. B) Validation experiment carried out in 10 healthy subjects and 10 CF patients before and after antibiotic treatment. Each measurement was made in triplicate and expressed relative to the detection of the housekeeping gene HPRT. For quantitative RT-PCR, statistics were performed by utilizing paired t-test. *P<0.05, post vs. pre.