Figure 5. Real time PCR data on HVCN1, PMAIP1, ARRB1 and DOM3Z in sputum neutrophils obtained from 6 CF patients before and after therapy.

Gene expression in CF neutrophils before therapy was set at 1. Each measurement was made in triplicate and expressed relative to the detection of the housekeeping gene HPRT. For quantitative RT-PCR, statistics were performed utilizing paired t-test. All four genes were up-regulated in airway PMN after therapy but did not reach statistically significance.