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. 2014 Aug 1;9(8):e103938. doi: 10.1371/journal.pone.0103938

Figure 2. Knockdown of CXCR7 by RNA interference.

Figure 2

HBMECs stably infected with CXCR7-targeted shRNA or scrambled control shRNA lentiviruses were subjected to qPCR, Western blot and flow cytometry analysis, respectively, as described in “Materials and Methods”. A, Total RNA from shRNA-CXCR7 and shRNA-scramble HBMECs was analyzed for CXCR7 by qPCR. Data are shown as means ± S.D. from three independent experiments. **, p<0.01. B. Total protein from shRNA-CXCR7 and shRNA-scramble HBMECs was analyzed by Western blot. Three independent experiments were performed and representative data are shown. C. shRNA-scramble and shRNA-CXCR7 HBMECs were collected from 6 well plates and subjected to flow cytometry analysis. Three independent experiments were performed and representative data are shown. D. Total RNA from shRNA-CXCR7 and shRNA-scramble HBMECs was analyzed for CXCR4 by qPCR. Data are shown as means ± S.D from three independent experiments. N.S., not significant. SC: shRNA-scramble HBMECs; p148: shRNA-CXCR7 HBMECs.