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. 2014 Aug 1;9(8):e103938. doi: 10.1371/journal.pone.0103938

Figure 3. CXCR7 regulates multiple functions in HBMECs.

Figure 3

A. shRNA-scramble CXCR7 or shRNA-CXCR7 HBMECs were seeded on 96 well plate in the presence of 200 ng/ml CXCL12 and cell proliferation was analyzed by XTT assay as described in “Materials and Methods”. B. shRNA-scramble or shRNA-CXCR7 HBMECs were seeded on 24 well plate coated with matrigel and tubulogenesis assay was performed as described in in “Materials and Methods”. C. shRNA-scramble CXCR7 or shRNA-CXCR7 HBMECs were seeded on the upper chamber of BD matrigel invasion chambers while the lower chamber was filled with 600 µl 10% complete medium with CXCL12 (200 ng/ml). Cell migration ability was analyzed as described in “Materials and Methods”. Data are shown as means ± S.D from three independent experiments. *, p<0.05; **, p<0.01; SC: shRNA-scramble HBMECs; p148: shRNA-CXCR7 HBMECs.