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. 2014 Aug 1;9(8):e103938. doi: 10.1371/journal.pone.0103938

Figure 4. CXCR7 regulates HBMEC adhesion and binding to GBM cells.

Figure 4

A. shRNA-scramble HBMECs or shRNA-CXCR7 HBMECs were seeded on matrigel or GBM cells (U251 and U373) and adhesion was measured as described in “Materials and Methods”. B, HBMECs were pretreated with CXCR7 specific antibody (11G8) or isotype-IgG1 for 30 min. and subsequent adhesion was measured as described in “Materials and Methods”. C. HBMECs were pretreated with a CXCR7 small molecule antagonist, CCX771, or negative control, CCX704, for 30 min. and adhesion assays were performed as described in “Materials and Methods”. Data are shown as means ± S.D from three independent experiments. **, p<0.01; N.S., not significant; SC: shRNA-scramble HBMECs; p148: shRNA-CXCR7 HBMECs.