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. Author manuscript; available in PMC: 2015 Aug 12.
Published in final edited form as: J Mol Biol. 2014 Jun 18;426(16):2970–2981. doi: 10.1016/j.jmb.2014.06.010

Figure 4. Rtr1 is a dual specificity phosphatase that acts both on Ser5 and Tyr1.

Figure 4

a) TFIIH efficiently phosphorylates the GST-CTD. With the exception of the anti-Ser7P antibody, all antibodies used recognize only the phosphorylated form of the CTD.

b) GST-CTD phosphorylated with either purified TFIIH (left) or TFIIH/Abl kinase (right) were used as substrates for KlRtr1. Reaction products were separated by SDS-PAGE and then western blots were then probed with antibodies against Ser2, Ser5, Ser7, and Tyr1. Two exposures for Tyr1 are shown for clarity.

c) Quantitation of phospho-signals from the Ser2P, Ser5P, Ser7P, and Tyr1P blots (n = 3) on GST-CTD modified by TFIIH or TFIIH plus Abl kinase.