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Steady state kinetic parameters of Rtr1 assayed against the synthetic substrate DiFMUP.
| Rtr1 construct | Km (μM) | Kcat (s−1) |
|---|---|---|
| Wild-type KlRtr1 FL | 587 ± 70 | 0.0010 ± 0.00003 |
| Wild-type KlRtr1 NTD | 694 ± 74 | 0.0015 ± 0.00005 |
| KlRtr1 CTR | Not detectable | Not detectable |
| KlRtr1 FL E66Q | Not detectable | Not detectable |
| KlRtr1 FL E197Q | 529 ± 45 | 0.0016 ± 0.00004 |
| hRPAP2 NTD | 1668 ± 205 | 0.0005 ± 0.00003 |
