Figure 4.

Detailed analysis of nad5 splicing. A, Diagram of the splicing events necessary for generating a translatable nad5 transcript and positions of the primers used. B, Quantitative RT-PCR of the mature and unspliced mRNAs of individual nad5 exons in tang2 and otp439 analyzed using various combinations of the primers described in A and in Supplemental Table S1. The histogram shows the log₂ values of the ratios of mutants to complemented plants for each transcript. Each value is a mean of three biological replicates.