. 2014 Jun 6;165(4):1575–1590. doi: 10.1104/pp.114.242610

Table I. Kinetic parameters of recombinant AtPAO5.

k_cat and K_m values were obtained from the Lineweaver-Burk plot analyses. Experiments were repeated at least three times and the mean ± sd values are displayed. ND, not determined.

Substrate	pH 7.5			pH 6.5
Substrate	k_cat	K_m	k_cat/K_m	k_cat	K_m	k_cat/K_m
	S⁻¹	μM	M⁻¹ s⁻¹	S⁻¹	μM	M⁻¹ s⁻¹
Spm	0.069 ± 0.003	25.56 ± 2.07	2,700 ± 142	0.009 ± 0.001	78.67 ± 7.13	115 ± 18
T-Spm	0.115 ± 0.007^a	5.09 ± 1.06^a	23,256 ± 3,299^a	0.035 ± 0.003	13.65 ± 1.50	2,596 ± 68
NorSpm	0.086 ± 0.006	25.54 ± 1.81	3,386 ± 111	0.012 ± 0.001	15.81 ± 0.64	747 ± 17
N¹-AcSpm	0.084 ± 0.004^a	1.92 ± 0.16^a	44,020 ± 1,943^a	0.025 ± 0.003^a	2.18 ± 0.57^a	12,323 ± 2,409^a
Spd	0.009 ± 0.002	68.30 ± 35.61	185 ± 98	ND	ND	ND

The reaction occurred in a noncanonical Michaelis-Menten fashion. In <10 μm substrate concentration, the reaction occurred linearly. In cases with >20 μm substrate concentration, each reaction was markedly repressed. In those cases, the values were calculated from the data with one-order reaction.